Journal: Marine Drugs

Article Title: Effect of Marine Microalga Chlorella pyrenoidosa Ethanol Extract on Lipid Metabolism and Gut Microbiota Composition in High-Fat Diet-Fed Rats

doi: 10.3390/md16120498

Figure Lengend Snippet: Effect of CPE55 on the mRNA and protein expressions levels in the liver. The mRNA expression ( A ) and protein expression ( B , C ) of Acetyl CoA carboxylase (ACC), AMPK-α, 3-Hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA), and Sterol regulatory element-binding transcription factor-1c (SREBP-1c) levels were determined through real-time quantitative PCR (RT-qPCR) and western blotting analysis. Data are expressed as the mean ± SD. One-way ANOVA with Tukey’s test. * p < 0.05 and ** p < 0.01 for CPE55 versus NFD; # p < 0.05, ## p < 0.01 for CPE55 versus HFD.

Article Snippet: The membranes were incubated for 3.5 h at 37 °C using rabbit polyclonal antibodies against GAPDH, HMG-CoA, SREBP-1c, ACC, and AMPK-α (1:1000; Beyotime Biotechnology, Shanghai, China).

Techniques: Expressing, Binding Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: High mobility group protein B1 (HMGB1) interacts with receptor for advanced glycation end products (RAGE) to promote airway smooth muscle cell proliferation through ERK and NF- κ B pathways

doi:

Figure Lengend Snippet: Effect of HMGB1 on proliferation of RASMCs. A. Representative western blot analysis for PCNA protein. The primary RASMCs were treated with the indicated concentrations of HMGB1 (100-1000 ng/ml) for 48 h. GAPDH was selected to normalize the PCNA level. *P < 0.05 vs. Control cells; #P < 0.05 vs the RASMCs treated with HMGB1 100 ng/ml. B. Effect of HMGB1 on RASMCs proliferation detected by the CCK-8 assay. The primary RASMCs were treated with HMGB1 for 48 h. *P < 0.05 vs. Control cells; #P < 0.05 vs. the RASMCs treated with HMGB1 100 ng/ml. C. The RASMCs were treated with HMGB1 1000 ng/ml for 0, 12, 24, 48 h. *: P < 0.05 vs. unstimulated cells at 0 h; #: P < 0.05 vs the RASMCs treated with HMGB1 for 12 h; &: P < 0.05 vs the RASMCs treated with HMGB1 for 24 h. D. Cell viability was detected by trypan blue staining. Data are expressed as mean ± SEM from four independent experiments.

Article Snippet: After 60 min of incubation at room temperature in 5% non-fat milk in TBST, the membranes were exposed to polyclonal Abs against GAPDH and PCNA (Immunoway, USA), rabbit monoclonal Abs against Erk1/2 and phosphorylated Erk1/2 (Cell Signaling Biotechnology) and rabbit monoclonal Abs against phosphorylated p65 (Cell Signaling Biotechnology).

Techniques: Western Blot, Control, CCK-8 Assay, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: High mobility group protein B1 (HMGB1) interacts with receptor for advanced glycation end products (RAGE) to promote airway smooth muscle cell proliferation through ERK and NF- κ B pathways

doi:

Figure Lengend Snippet: The effects of related receptors on HMGB1-induced proliferation of RASMCs. The primary RASMCs were pretreated with Cu-CPT22 (50 nM), CLI-095 (1 ug/ml) and FPS-ZMI (230 nM) for 1 hour, followed by stimulation with HMGB1 1000 ng/ml for 48 h. A. Cell proliferation was evaluated by a CCK-8 assay. B. The PCNA protein expression was determined by western blot and GAPDH was used as the internal control. *: P < 0.05 vs. unstimulated cells; #: P < 0.05 vs. the RASMCs treated with HMGB1. Data are expressed as mean ± SEM from four independent experiments.

Article Snippet: After 60 min of incubation at room temperature in 5% non-fat milk in TBST, the membranes were exposed to polyclonal Abs against GAPDH and PCNA (Immunoway, USA), rabbit monoclonal Abs against Erk1/2 and phosphorylated Erk1/2 (Cell Signaling Biotechnology) and rabbit monoclonal Abs against phosphorylated p65 (Cell Signaling Biotechnology).

Techniques: CCK-8 Assay, Expressing, Western Blot, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: High mobility group protein B1 (HMGB1) interacts with receptor for advanced glycation end products (RAGE) to promote airway smooth muscle cell proliferation through ERK and NF- κ B pathways

doi:

Figure Lengend Snippet: Effects of specific signaling pathway inhibitors on HMGB1-induced proliferation of RASMCs. The primary RASMCs were incubated for 1 h with or without the protein kinase inhibitors BAY11-7082 (10 µM), U0126 (10 µM), SB203580 (10 µM) or SP600125 (50 µM) before stimulation with HMGB1 (1,000 ng/ml) for 48 h. A. The cell proliferation was evaluated by a CCK-8 assay. B. The PCNA protein expression was determined by western blot and GAPDH was used as the internal control. *: P < 0.05 vs. unstimulated cells; #: P < 0.05 vs. the RASMCs treated with HMGB1. Data are expressed as mean ± SEM from four independent experiments.

Article Snippet: After 60 min of incubation at room temperature in 5% non-fat milk in TBST, the membranes were exposed to polyclonal Abs against GAPDH and PCNA (Immunoway, USA), rabbit monoclonal Abs against Erk1/2 and phosphorylated Erk1/2 (Cell Signaling Biotechnology) and rabbit monoclonal Abs against phosphorylated p65 (Cell Signaling Biotechnology).

Techniques: Incubation, CCK-8 Assay, Expressing, Western Blot, Control